Human ER alpha/NR3A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB57152

R&D Systems, part of Bio-Techne
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MAB57152
MAB57152-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 681104

View all ER alpha/NR3A1 Antibodies »

Product Summary for Human ER alpha/NR3A1 Antibody

Immunogen

E. coli-derived recombinant human ER alpha/NR3A1
Thr2-Gln116
Accession # P03372

Specificity

Detects human ER alpha/NR3A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ER beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ER alpha/NR3A1 Antibody

ERa/NR3A1 antibody in Human Breast by Immunohistochemistry (IHC-P).

ER alpha/NR3A1 in Human Breast.

ERa/NR3A1 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human ERa/NR3A1 Monoclonal Antibody (Catalog # MAB57152) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ER alpha/NR3A1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast  
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ER alpha/NR3A1

ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins.  It is widely expressed, and serves as a strong activator of estrogen-responsive genes.  ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins.  Following beta-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple coactivators.  Human ER alpha is 595 amino acids (aa) in length.  It contains a DNA binding region (aa 185-250), three NLSs (aa 256-260; 266-271; 299-303), a steroid-binding site (aa 351-543), a dimerization motif (aa 497-518), and an O-GlcNAc attachment around Thr575.  Major phosphorylation sites exist at Tyr537, Ser167 and Ser118.  Multiple splice forms exist.  There is an 80 kDa isoform that shows a substitution (duplication) of aa 412-517 for Asp411, a second isoform with a deletion of aa 255-366, a third isoform with a deletion of aa 152-412, and a fourth isoform that shows a Thr substitution for aa 152-595.  Human ER alpha is only 46% aa identical to human ER beta.  Over aa 1-116, human ER alpha shares 85% aa identity with mouse ER alpha.

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human)

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for Human ER alpha/NR3A1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ER alpha/NR3A1 Antibody

For research use only

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