Human ERMAP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4928
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 767504
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ERMAP
His30-Ala155
Accession # Q96PL5
His30-Ala155
Accession # Q96PL5
Specificity
Detects human ERMAP in direct ELISAS.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human ERMAP Antibody
Detection of Human ERMAP by Western Blot.
Western blot shows lysates of human erythrocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ERMAP Monoclonal Antibody (Catalog # MAB4928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ERMAP at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human ERMAP Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human erythrocytes
Sample: Human erythrocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERMAP
ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60‑66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30‑155) that contains one V-type Ig-like domain (aa 30‑140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103‑475. Over aa 30‑155, human ERMAP is 56% aa identical to mouse ERMAP. In contrast to human ERMAP ECD, mouse ERMAP ECD contains one extra Ig-like domain.
Long Name
Erythroblast Membrane-associated Protein
Alternate Names
RD, SC
Gene Symbol
ERMAP
UniProt
Additional ERMAP Products
Product Documents for Human ERMAP Antibody
Product Specific Notices for Human ERMAP Antibody
For research use only
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