Human ERp57/PDIA3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8219
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant ERp57/PDIA3
Arg107-Lys366
Accession # P30101
Arg107-Lys366
Accession # P30101
Specificity
Detects human ERp57/PDIA3 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ERp57/PDIA3 Antibody
Detection of Human and Mouse ERp57/PDIA3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ERp57/PDIA3 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ERp57/PDIA3 in HeLa Human Cell Line.
ERp57/PDIA3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ERp57/PDIA3 in Human Liver.
ERp57/PDIA3 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human ERp57/PDIA3 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and Hepa 1‑6 mouse hepatoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and Hepa 1‑6 mouse hepatoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERp57/PDIA3
ERp57 (Endoplasmic reticulum resident protein 57; also Protein disulfide-isomerase A3/PDIA3) is a 57-60 kDa member of the protein disulfide isomerase family of proteins. Knockdown of ERp57 or antibody-targeted inhibition of the secreted form significantly impaired the secretion and accumulation of extracellular matrix. ERp57 is highly similar to other PDI family members in amino acid sequence and structural/functional domain organization. This protein plays an important role in endoplasmic reticulum quality control of newly synthesized glycoproteins, is critical in major histocompatability complex class I assembly and regulates gene expression. ERp57 has been implicated in human pathologies including cancer and Alzheimer's disease. Within aa 107 - 366, human ERp57 shares 92% aa sequence identity with mouse ERp57.
Long Name
Endoplasmic Reticulum Resident Protein 57
Alternate Names
ERp60, GRP58, PDIA3
Gene Symbol
PDIA3
UniProt
Additional ERp57/PDIA3 Products
Product Documents for Human ERp57/PDIA3 Antibody
Product Specific Notices for Human ERp57/PDIA3 Antibody
For research use only
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