Human F-box protein 15/FBXO15 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6035
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human F‑box protein 15/FBXO15
aa 298-434
Accession # NP_689889
aa 298-434
Accession # NP_689889
Specificity
Detects human F‑box protein 15/FBXO15 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse F‑box protein 15/FBXO15 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human F-box protein 15/FBXO15 Antibody
Detection of Human F-box protein 15/FBXO15 by Western Blot.
Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human F-box protein 15/FBXO15 Antibody Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6035) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for F-box protein 15/FBXO15 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human F-box protein 15/FBXO15 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human testis tissue
Sample: Human testis tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: F-box protein 15/FBXO15
FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site, and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15.
Alternate Names
Fbox protein 15, FBX15, FBXO15
Gene Symbol
FBXO15
UniProt
Additional F-box protein 15/FBXO15 Products
Product Documents for Human F-box protein 15/FBXO15 Antibody
Product Specific Notices for Human F-box protein 15/FBXO15 Antibody
For research use only
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