Human FABP7/B-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3166
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate - Macaca fuscata (Japanese Macaque)
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FABP7/B-FABP
Met1-Ala132
Accession # O15540
Met1-Ala132
Accession # O15540
Specificity
Detects human FABP7/B-FABP in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP6, rhFABP8, rhFABP9 and recombinant mouse FABP9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human FABP7/B-FABP Antibody
FABP7 in Human Melanoma.
FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human FABP7/B‑FABP by Simple WesternTM.
Simple Western lane view shows lysates of human cerebellum tissue and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP7/B-FABP at approximately 18 kDa (as indicated) using 50 µg/mL of Goat Anti-Human FABP7/B-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human FABP7/B-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma and astrocytoma
Sample: Immersion fixed paraffin-embedded sections of human melanoma and astrocytoma
Simple Western
50 µg/mL
Sample: Human cerebellum tissue and human hippocampus tissue
Sample: Human cerebellum tissue and human hippocampus tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human FABP7
Sample: Recombinant Human FABP7
Reviewed Applications
Read 2 reviews rated 4 using AF3166 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP7/B-FABP
Long Name
Fatty Acid-Binding Protein 7
Alternate Names
B-FABP, BFABP, BLBP
Gene Symbol
FABP7
UniProt
Additional FABP7/B-FABP Products
Product Documents for Human FABP7/B-FABP Antibody
Product Specific Notices for Human FABP7/B-FABP Antibody
For research use only
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