Human FAM3C Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3084
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 438618
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FAM3C
Gln25-Asp227
Accession # Q92520
Gln25-Asp227
Accession # Q92520
Specificity
Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, recombinant mouse (rm) FAM3C, or rmFAM3D is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FAM3C Antibody
FAM3C in A549 Human Cell Line.
FAM3C was detected in immersion fixed A549 human lung carcinoma cells treated with 10ng/mL of Recombinant Human TGF-beta 1 (Catalog # 240-B) for 48 hours using Mouse Anti-Human FAM3C Monoclonal Antibody (Catalog # MAB3084) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human FAM3C Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cells treated with 10ng/mL of Recombinant Human TGF-beta 1 (Catalog # 240-B)
Sample: Immersion fixed A549 human lung carcinoma cells treated with 10ng/mL of Recombinant Human TGF-beta 1 (Catalog # 240-B)
Western Blot
1 µg/mL
Sample: Recombinant Human FAM3C
Sample: Recombinant Human FAM3C
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FAM3C
FAM3C (Family with sequence similarity 3, member C), also known as D6WSU176e, is a secreted protein belonging to a cytokine-like family. Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. FAM3C is ubiquitously expressed in multiple tissues. The human FAM3C gene has been identified to be a candidate gene for autosomal recessive non-syndromic hearing loss locus 17. The amino acid sequence of mature mouse FAM3C is 97% and 94% identical to that of rat and human FAM3C, respectively.
Long Name
Family With Sequence Similarity 3, Member C
Alternate Names
D6WSU176e, ILEI
Gene Symbol
FAM3C
UniProt
Additional FAM3C Products
Product Documents for Human FAM3C Antibody
Product Specific Notices for Human FAM3C Antibody
For research use only
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