Human Fc gamma RIII (CD16) Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2546F

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 245536

View all Fc gamma RIII (CD16) Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)
Thr20-Gln208
Accession # O75015

Specificity

Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Fc gamma RIII (CD16) Fluorescein-conjugated Antibody

Detection of Fc gamma RIII (CD16) antibody in Human Blood Neutrophils antibody by Flow Cytometry.

Detection of Fc gamma RIII (CD16) in Human Blood Neutrophils by Flow Cytometry.

Human peripheral blood neutrophils were stained with Mouse Anti-Human Fc gamma RIII (CD16) Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2546F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Fc gamma RIII (CD16) Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood neutrophils
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Long Name

Fc gamma Receptor III

Alternate Names

FcgRIII

Entrez Gene IDs

14131 (Mouse); 102140945 (Cynomolgus Monkey)

Gene Symbol

FCGR3

UniProt

O75015

Additional Fc gamma RIII (CD16) Products

Product Documents for Human Fc gamma RIII (CD16) Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fc gamma RIII (CD16) Fluorescein-conjugated Antibody

For research use only

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