Human Fc gamma RII/CD32 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1330P

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 190723

View all Fc gamma RII/CD32 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB
Ala46-Pro217
Accession # P31994

Specificity

Detects human Fc gamma RIIA and human Fc gamma RIIB in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse Fc gamma RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Fc gamma RII/CD32 PE-conjugated Antibody

Detection of Fc? RII/CD32 antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of Fc gamma RII/CD32 in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human Fc gamma RII/CD32 PE-conjugated Monoclonal Antibody (Catalog # FAB1330P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Fc gamma RII/CD32 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood granulocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RII/CD32

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T cells, and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name

Fc gamma Receptor II

Alternate Names

FcgRII

Entrez Gene IDs

2212 (Human); 14130 (Mouse)

Gene Symbol

FCGR2A

UniProt

P31994

Additional Fc gamma RII/CD32 Products

Product Documents for Human Fc gamma RII/CD32 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fc gamma RII/CD32 PE-conjugated Antibody

For research use only

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