Human Fc gamma RIIB/C (CD32b/c) Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1875

R&D Systems, part of Bio-Techne
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BAM1875
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Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # 190703

View all Fc gamma RIIB/C (CD32b/c) Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB/C
Ala46-Pro217
Accession # P31994

Specificity

Detects human Fc gamma RIIB/C in ELISAs. In ELISAs, this antibody shows approximately 40% cross-reactivity with recombinant human (rh) Fc gamma RIIA and no cross‑reactivity with rhFc gamma RI, rmFc gamma RI, rmFc gamma RIIB, rhFc gamma RIIIB, rmFc gamma RIII, or rmFcrl3/CD16-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Fc gamma RIIB/C (CD32b/c) Biotinylated Antibody

Application
Recommended Usage

Human Fc gamma RIIB/CD32b Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Fc gamma RIIB/C (CD32b/c) Antibody (Catalog # MAB18751)
  • Standard: Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF (Catalog # 1875-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIB/C (CD32b/c)

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name

Fc gamma Receptor IIB/C

Alternate Names

CD32;CD32B;FCG2;FcgammaRIIb;FCGR2;FCGR2B;FCGR2C;FcGRIIB;FcRII-c;IGFR2;Low affinity immunoglobulin gamma Fc region receptor II-b

Entrez Gene IDs

2213 (Human); 14130 (Mouse)

Gene Symbol

FCGR2B

UniProt

P31994

Additional Fc gamma RIIB/C (CD32b/c) Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fc gamma RIIB/C (CD32b/c) Biotinylated Antibody

For research use only

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