Human FCRL1/FcRH1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2049

R&D Systems, part of Bio-Techne
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AF2049
AF2049-SP
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BAF2049
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all FCRL1/FcRH1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FCRL1/FcRH1
Ala17-His304
Accession # Q96LA6

Specificity

Detects human FCRL1/FcRH1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ITRA4 is observed, and less than 5% cross‑reactivity with rhIRTA3, rhIRTA2, and rhIRTA1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human FCRL1/FcRH1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human FCRL1/FcRH1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL1/FcRH1

Fc Receptor-Like 1 (FCRL1), also known as FcRH1, and IRTA5, is an approximately 50 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL1 binds to purified human IgG. Mature human FCRL1 consists of a 291 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 101 aa cytoplasmic domain with two immunotyrosine activation motifs (ITIMs) (3, 4). A charged glutamic acid within the transmembrane segment may mediate association with other signaling proteins. Alternative splicing may generate an isoform that lacks the transmembrane segment and an isoform that largely consists of the first two Ig-like domains (5). Mouse FCRL1 contains only two Ig-like domains, but it shares 62% aa sequence identity with homologous regions of the human FCRL1 ECD. FCRL1 is expressed on pre-B cells and naive B cells (6, 7). It is down‑regulated upon B cell activation but up‑regulated on memory B cells (6, 8). FCRL1 is expressed on many B cell lymphoma and leukemia tumor cells with the exception of B cell acute lymphoblastic leukemia (9‑11). Antibody crosslinking of FCRL1 triggers its tyrosine phosphorylation and augments B cell proliferation induced by the B cell receptor (6).

References

  1. Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Miller, I. et al. (2002) Blood 99:2662.
  4. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
  5. Accession # Q96LA6.
  6. Leu, C.-M. et al. (2005) Blood 105:1121.
  7. Li, F.J. et al. (2008) Blood 112:179.
  8. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  9. Du, X. et al. (2008) Blood 111:338.
  10. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
  11. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.

Long Name

Fc Receptor-like 1

Alternate Names

BXMH1, CD307a, FcRH1, IFGP1, IRTA5

Entrez Gene IDs

115350 (Human); 229499 (Mouse); 680665 (Rat)

Gene Symbol

FCRL1

UniProt

Q96LA6

Additional FCRL1/FcRH1 Products

Product Documents for Human FCRL1/FcRH1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FCRL1/FcRH1 Antibody

For research use only

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