Human FCRLA Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7965

R&D Systems, part of Bio-Techne
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AF7965
AF7965-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all FCRLA Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FCRLA
Pro270-Glu359
Accession # Q7L513

Specificity

Detects human FCRLA in direct ELISAs and Western blots. In direct ELISAs, approximately 5-10% cross-reactivity with recombinant mouse FCRLA is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FCRLA Antibody

Detection of Human FCRLA antibody by Western Blot.

Detection of Human FCRLA by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human FCRLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7965) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FCRLA at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FCRLA Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRLA

FCRLA (Fc Receptor-Like A; also known as FcRX, FcRL1 and Fc receptor Homolog Expressed in B cells/FREB) is a 40-44 kDa intracellular member of the Fc gammaRI class, FcR family, Immunoglobulin Superfamily of molecules. Initially, it is associated with germinal center B cells that are undergoing somatic hypermutation and class-switch recombination. Now, it is believed that all B cells (but not CD38+ plasma cells) are also FCRLA+. FCRLA is suggested to act as an ER chaperone during antibody maturation, and is known to bind to IgM, IgA, and IgG prior to their secretion. Human FCRLA is synthesized as a 359 amino acid (aa) precursor that contains a 27 aa signal sequence plus a mature region that contains two C2-type Ig-like domains (aa 70-159 and 170-257), and a C-terminal poly-Proline region (aa 269-315). Although there is no traditional ER retention signal, a viable substitute is assumed to exist in the N-terminus of the mature molecule. FCLRA is believed to exist naturally as a monomer; however, disulfide-linkage can occur during experimental manipulation. At least eight potential isoform variants have been reported. It is unclear if, or how frequently they are expressed. One utilizes an alternative start site 17 aa upstream of the standard site, a second contains a six aa insertion after Ala28, and a third possessess an four aa substitution for aa 29-166. Five show single block deletions; in random order, they involve aa 78-166, 27-166, 78-261, 21‑261 and 167-26. Over aa 270-359, human FCLRA shares 55% aa sequence identity with mouse FCRLA.

Long Name

Fc Receptor-like A

Alternate Names

FCRLc1, FCRLM1, FCRLX, FcRX, FREB

Entrez Gene IDs

84824 (Human); 98752 (Mouse)

Gene Symbol

FCRLA

UniProt

Q7L513

Additional FCRLA Products

Product Documents for Human FCRLA Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FCRLA Antibody

For research use only

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