Human Ferritin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB93541
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 962609
Product Specifications
Immunogen
Human Ferritin synthetic peptide
Accession # P02794
Accession # P02794
Specificity
Detects human Ferritin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Ferritin Antibody
Detection of Human Ferritin by Western Blot.
Western blot shows lysates of human liver tissue and human spleen tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Ferritin Monoclonal Antibody (Catalog # MAB93541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ferritin at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Ferritin in iBJ6 iPS Cell Line.
Ferritin was detected in immersion fixed iBJ6 iPS cell line differentiated into hepatocytes using Mouse Anti-Human Ferritin Monoclonal Antibody (Catalog # MAB93541) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Ferritin Antibody
Application
Recommended Usage
Immunocytochemistry
1 µg/mL
Sample: Immersion fixed iBJ6 iPS cell line differentiated into hepatocytes
Sample: Immersion fixed iBJ6 iPS cell line differentiated into hepatocytes
Western Blot
5 µg/mL
Sample: Human liver tissue and human spleen tissue
Sample: Human liver tissue and human spleen tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB93541 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ferritin
Alternate Names
apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTHEC 1.16.3.1, FTHL6MGC104426, PIG15, placenta immunoregulatory factor, PLIF, proliferation-inducing protein 15
Entrez Gene IDs
2495 (Human)
Gene Symbol
FTH1
UniProt
Additional Ferritin Products
Product Documents for Human Ferritin Antibody
Product Specific Notices for Human Ferritin Antibody
For research use only
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