Human FFAR1/GPR40 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11025
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1048036
Product Specifications
Immunogen
Synthetic peptide within aminoacids 1-50 of human FFAR1/GPR40
Accession # O14842
Accession # O14842
Specificity
Detects human FFAR1/GPR40 in ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FFAR1/GPR40 Antibody
Detection of FFAR1/GPR40 in Human Brain Hypothalamus.
FFAR1/GPR40 was detected in immersion fixed paraffin-embedded sections of human brain hypothalamus using Mouse Anti-Human FFAR1/GPR40 Monoclonal Antibody (Catalog # MAB11025) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human FFAR1/GPR40 Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: paraffin-embedded sections of human hypothalamus
Sample: paraffin-embedded sections of human hypothalamus
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FFAR1/GPR40
Human Free Fatty Acid Receptor 1 (FFAR-1), also known as GPR40, is a class A G-protein coupled receptor (GPCR) for medium and long chain saturated and unsaturated fatty acids encoded by the FFAR1 gene. FFAR-1 is expressed in pancreas and it plays an important role in glucose homeostasis acting as a nutrient sensor. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1).
References
Long Name
Free Fatty Acid Receptor 1
Alternate Names
FFA1, GPR40
Gene Symbol
FFAR1
UniProt
Additional FFAR1/GPR40 Products
Product Documents for Human FFAR1/GPR40 Antibody
Product Specific Notices for Human FFAR1/GPR40 Antibody
For research use only
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