Human FGF-17 Antibody

Name: Human FGF-17 Antibody
Brand: R&D Systems
SKU: AF319

R&D Systems, part of Bio-Techne | Catalog # AF319

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Product Datasheet / COA / SDS

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Conjugate

Catalog #

Biotin

BAF319

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all FGF-17 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-17
Thr23-Thr216
Accession # O60258

Specificity

Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) FGF-8b and recombinant mouse FGF-8c is observed and less than 2% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF‑4, rhFGF‑5, rhFGF‑6, rhFGF‑7, rhFGF‑10, and rhFGF‑18 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human FGF-17 Antibody

Cell Proliferation Induced by FGF-17 and Neutralization by Human FGF-17 Antibody.

Recombinant Human FGF-17 (Catalog # 319-FG) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-17 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF319). The ND₅₀ is typically 6-24 µg/mL in the presence of heparin (1 µg/mL).

Applications for Human FGF-17 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human FGF-17 (Catalog # 319-FG)

Neutralization

Measured by its ability to neutralize FGF-17-induced proliferation in the NR6R-3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND₅₀) is typically 6-24 µg/mL in the presence of 100 ng/mL Recombinant Human FGF-17 and 1 µg/mL heparin.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-17

Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30‑70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF‑17 shares 98.6% amino acid (aa) sequence identity with mouse FGF‑17. Rat FGF‑17 shares 100% aa sequence identity with mouse FGF‑17.

References

Hoshikawa, M. et al. (1998) Biochem. Biophys. Res. Commun. 244:187.
Xu, J. et al. (1999) Mech. Dev. 83:165.

Long Name

Fibroblast Growth Factor 17

Alternate Names

FGF17

Entrez Gene IDs

8822 (Human); 14171 (Mouse)

Gene Symbol