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Human FGF-4 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF235

R&D Systems, part of Bio-Techne
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BAF235

Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair), Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-4
Ala31-Leu206
Accession # P08620

Specificity

Detects human FGF-4 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) FGF‑6 is observed, and less than 0.2% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF-5, -7, -9, -10, -17, and -19 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human FGF-4 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Western Blot

0.1 µg/mL
Sample: Recombinant Human FGF-4 (Catalog # 235-F4)

Human FGF-4 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human FGF-4 Antibody (Catalog # MAB635)
  • Standard: Recombinant Human FGF-4 Protein (Catalog # 235-F4)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-4

FGF-4, the product of a developmentally regulated gene (hst-1), is a member of the FGF family that is efficiently secreted. The gene for FGF-4 (also known as FGFK or K-FGF for Kaposi sarcoma-associated FGF) was initially discovered as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors (including stomach and colon cancers, hepatocellular carcinomas, and Kaposi’s sarcomas). FGF-4 does not seem to be expressed in normal adult tissues. However, expression of the gene is spatially and temporally regulated during embryonic development. The murine homologue of human FGF-4 has been cloned and shown to be 82% homologous to the human protein at the amino acid sequence level. Human FGF-4 has been shown to exhibit cross species activity.

In vitro, FGF-4 is mitogenic for fibroblasts and endothelial cells. FGF-4 has been shown to be a potent angiogenesis promoter in vivo. FGF-4 has potent transforming potential apparently through an autocrine mechanism of action. FGF-4 plays a key role in limb development and has been identified as the molecular mediator of the activities of the apical ectodermal ridge that is required for directing the outgrowth and patterning of vertebrate limbs.

Long Name

Fibroblast Growth Factor 4

Alternate Names

FGF4, HBGF-4, HST-1, KFGF, KS3

Entrez Gene IDs

2249 (Human); 14175 (Mouse); 116499 (Rat)

Gene Symbol

FGF4

UniProt

Additional FGF-4 Products

Product Documents for Human FGF-4 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF-4 Biotinylated Antibody

For research use only

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