Human FGFR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6843
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Neutralization
Cited:
Immunocytochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 98739
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms
Specificity
Detects all isoforms of human FGF R2 ( alpha and beta, IIIb and IIIc). In direct ELISAs, approximately 25‑50% cross-reactivity with mouse FGF R2 and no cross-reactivity with any isoform of recombinant human (rh) FGF R1, rhFGF R3, or rhFGF R4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human FGFR2 Antibody
FGF R2 in Human Adenocarcinoma.
FGF R2 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Mouse Anti-Human FGF R2 Monoclonal Antibody (Catalog # MAB6843) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.
Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 beta (IIIc) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB6843). The ND50 is typically 0.1-0.5 µg/mL in the presence of heparin (10 µg/mL).Applications for Human FGFR2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Kato III human cell line
Sample: Kato III human cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue and human adenocarcinoma
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue and human adenocarcinoma
Neutralization
Measured by its ability to neutralize FGF R2 beta-mediated inhibition of proliferation in the NR6R-3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R2 beta (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGFR2
Fibroblast Growth Factor Receptor 2 (FGF R2) is one of four closely related transmembrane tyrosine kinases (FGF R1‑4) that function as receptors for the fibroblast growth factor family. Multiple isoforms are generated by alternative mRNA splicing resulting in extracellular domains with three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. In addition, alternative exon usage in the Ig III (membrane proximal) domain results in IIIb or IIIc isoforms.
Long Name
Fibroblast Growth Factor Receptor 2
Alternate Names
CD332, FGF R2
Gene Symbol
FGFR2
Additional FGFR2 Products
Product Documents for Human FGFR2 Antibody
Product Specific Notices for Human FGFR2 Antibody
For research use only
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