Human Fibrinogen Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4786

R&D Systems, part of Bio-Techne
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AF4786
AF4786-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Simple Western, Western Blot

Cited:

ELISA Development (Detection)

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Fibrinogen Antibodies »

Product Specifications

Immunogen

Human serum-derived Fibrinogen

Specificity

Detects human Fibrinogen in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Fibrinogen Antibody

Detection of Human Fibrinogen antibody by Western Blot.

Detection of Human Fibrinogen by Western Blot.

Western blot shows purified human fibrinogen. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Fibrinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Fibrinogen at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Fibrinogen antibody by Simple WesternTM.

Detection of Human Fibrinogen by Simple WesternTM.

Simple Western lane view shows human plasma, loaded at 1:25000. Specific bands were detected for Fibrinogen at approximately 64 kDa and 80 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Fibrinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4786) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Fibrinogen Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Human Plasma

Western Blot

0.1 µg/mL
Sample: Purified human fibrinogen

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibrinogen

Fibrinogen is a 340 kDa, secreted glycoprotein complex that is found in blood at concentrations of 150-400 mg/dL. It is secreted primarily by hepatocytes, but is also reported to be expressed by fibroblasts, type I alveolar epithelium, intestinal epithelium and some tumor cells. Fibrinogen is a homodimer that is composed of two, three-polypeptide chain subunits. In human, each subunit contains one 847amino acid (aa) alpha chain, one 461 aa beta chain, and one 427 aa gamma chain. Multiple interchain disulfide bonds link all three polypeptides. Fibrinogen plays a central role in clot formation. Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Fibrinogen is also a component of the ECM and binds to cell surface molecules on inflammatory cells. Mature human alpha, beta and gamma -chains share 67%, 85% and 83% aa identity with mouse alpha, beta and gamma -chains, respectively.

Alternate Names

Factor I

Entrez Gene IDs

2244 (Human); 14161 (Mouse); 361969 (Rat)

Gene Symbol

FGB

Additional Fibrinogen Products

Product Documents for Human Fibrinogen Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fibrinogen Antibody

For research use only

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