Human FOLR4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6328
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Binding Assay, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 821221
Product Specifications
Immunogen
E. coli-derived recombinant human FOLR4
Glu134-Trp210
Accession # A6ND01
Glu134-Trp210
Accession # A6ND01
Specificity
Detects human FOLR4 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human FOLR4 Antibody
FOLR4 in Human Melanoma.
FOLR4 was detected in immersion fixed paraffin-embedded sections of human melanoma using Rat Anti-Human FOLR4 Monoclonal Antibody (Catalog # MAB6328) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human FOLR4 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma
Sample: Immersion fixed paraffin-embedded sections of human melanoma
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOLR4
FOLR4 (Folate Receptor 4), also known as FR4, Folate Receptor delta, and Folate Binding Protein 3, is a 34‑36 kDa member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4hiCD25hi expression defines T reg cells, FR4loCD25+ expression pattern identifies effector memory T cells, and a FR4hiCD25- combination characterizes central memory T cells. Mature human FOLR4 is 225 amino acids (aa) in length (aa 20‑244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for aa 111‑116. Over aa 134‑220, human FOLR4 shares 72% aa identity with mouse FOLR4.
Long Name
Folate Receptor 4
Alternate Names
FR-delta, LOC390243
Gene Symbol
IZUMO1R
UniProt
Additional FOLR4 Products
Product Documents for Human FOLR4 Antibody
Product Specific Notices for Human FOLR4 Antibody
For research use only
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