Human FoxH1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4248
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FoxH1
Ser164-Ser272
Accession # O75593
Ser164-Ser272
Accession # O75593
Specificity
Detects human FoxH1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxB2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human FoxH1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human FoxH1
Sample: Recombinant Human FoxH1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxH1
FoxH1 (also known as FAST-1) is a 39 kDa, class 2, subfamily H member of the FOX (forkhead box) family of transcription factors. It is a nuclear protein that binds DNA promoter sites. It does not independently regulate transcription but interacts with SMAD2/4 to activate, and SMAD3/4 to inhibit gene transcription. Human FOXH1 is 365 amino acids (aa) in length. It contains an NLS (aa 22‑30), one FKH (fork head) DNA-binding domain (aa 32‑128) and a SMAD-interaction region (aa 273‑354). Over aa 164‑272, human FOXH1 is 55% identical to mouse FoxH1.
Long Name
Forkhead Box J1
Alternate Names
FAST1
Entrez Gene IDs
8928 (Human)
Gene Symbol
FOXH1
UniProt
Additional FoxH1 Products
Product Documents for Human FoxH1 Antibody
Product Specific Notices for Human FoxH1 Antibody
For research use only
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