Human FoxO1/FKHR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5939
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 597554
Product Specifications
Immunogen
E. coli- derived recombinant human FoxO1/FKHR
Ala353-Gly655
Accession # Q12778
Ala353-Gly655
Accession # Q12778
Specificity
Detects human FoxO1/FKHR in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FoxA1 (aa 253-472), B1 (aa 191-324), C1 (aa 208-322), D3 (aa 1-140), F1 (aa 129-354), G1 (aa 283-489), J1 (aa 301-421), J3 (aa 375-552), K1 (aa 493-670), M1 (aa 642-763), O3A (aa 372-673), P1 (aa 231-420), P2 (aa 269-460), P3 (aa 179-279), or P4 (aa 229-410) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FoxO1/FKHR Antibody
FoxO1/FKHR in LNCaP Human Cell Line.
FoxO1/FKHR was detected in immersion fixed LNCaP human prostate cancer cell line using Human FoxO1/FKHR Monoclonal Antibody (Catalog # MAB5939) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of FoxO1/FKHR in Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT-4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells.
FoxO1/FKHR was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT-4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells using Mouse Anti-Human FoxO1/FKHR Monoclonal Antibody (Catalog # MAB5939) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human FoxO1/FKHR Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line, Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells
Sample: Immersion fixed LNCaP human prostate cancer cell line, Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxO1/FKHR
Long Name
Forkhead Box O1
Alternate Names
FKH1, FKHR
Gene Symbol
FOXO1
UniProt
Additional FoxO1/FKHR Products
Product Documents for Human FoxO1/FKHR Antibody
Product Specific Notices for Human FoxO1/FKHR Antibody
For research use only
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