HEK293 human embryonic kidney cell line transfected with (A) human FSH R or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591) followed by APC-conjugated Goat anti-Mouse Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

cAMP Production Induced by FSH alpha/ beta and Neutralization by Human FSH R Antibody.

Recombinant Human FSH a/ beta (Catalog # 5925-FS) induces cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R in a dose-dependent manner (orange line), as measured by the cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recom-binant Human FSH a/ beta (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591). The ND₅₀ is typically 0.02-0.1 µg/mL.

Applications for Human FSHR Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human FSH R and eGFP

Neutralization

Measured by its ability to neutralize FSH alpha/ beta-induced cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The Neutralization Dose (ND₅₀) is typically 0.02-0.1 µg/mL in the presence of 1 ng/mL Recombinant Human FSH alpha/ beta.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FSHR

Follicle stimulating hormone receptor (FSH R), also called follitropin receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.

Long Name

Follicle Stimulating Hormone Receptor

Alternate Names

FSH R, LGR1, ODG1

Entrez Gene IDs

2492 (Human); 14309 (Mouse)

Gene Symbol

FSHR

UniProt

M65085

Additional FSHR Products

Product Documents for Human FSHR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FSHR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FSHR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB65591

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all FSHR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human FSHR Antibody

Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry.