Human Fucosyltransferase 3/5 (FUT3/5) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4068
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 425037
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fucosyltransferase 3
Arg35-Thr361
Accession # P21217
Arg35-Thr361
Accession # P21217
Specificity
Detects human Fucosyltransferase 3/5 (FUT3/5) in Western blots. The amino acid sequences of recombinant human FUT3 and recombinant human FUT5 are 91% identical.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Fucosyltransferase 3/5 (FUT3/5) Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT)
Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # 4949-GT)
Sample: Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT)
Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # 4949-GT)
Reviewed Applications
Read 2 reviews rated 3.5 using MAB4068 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fucosyltransferase 3/5 (FUT3/5)
Fucosyltransferase 3 (FUT3), also known as galactoside 3(4)-L-fucosyltransferase or blood group Lewis alpha-4-fucosyltransferase (Lewis FT), is type II membrane protein with a cytoplasmic tail (aa 1 to 15), a transmembrane region (aa 16 to 34) and an ectodomain (aa 35 to 361). The amino acid sequence of human FUT3 is 99% and 52% identical to that of chimpanzee and chicken.
Alternate Names
CD174, FT3B, FucT-III, LE, Les
Entrez Gene IDs
2525 (Human)
Gene Symbol
FUT3
UniProt
Additional Fucosyltransferase 3/5 (FUT3/5) Products
Product Documents for Human Fucosyltransferase 3/5 (FUT3/5) Antibody
Product Specific Notices for Human Fucosyltransferase 3/5 (FUT3/5) Antibody
For research use only
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