Human FXR/NR1H4 Antibody

R&D Systems, part of Bio-Techne | Catalog # PP-A9033A-00

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Direct ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # A9033A

View all FXR/NR1H4 Antibodies »

Product Specifications

Immunogen

Recombinant human FXR/NR1H4
aa 2-126

Specificity

This antibody specifically recognizes human FXR and cross-reacts with rat and mouse FXR. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human FXR/NR1H4 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.2 μg/mL with the appropriate secondary reagents to detect human FXR.

Immunohistochemistry

This antibody can be used at 20-40 μg/mL with the appropriate secondary reagents to detect human FXR.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human FXR.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using PP-A9033A-00 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: FXR/NR1H4

Farnesoid X-activated Receptor (FXR, HRR-1, BAR, RIP14; NR1H4) is a member of the Orphan Nuclear Receptor family. FXR is expressed in the liver, intestinal villi, renal tubes and adrenal cortex. FXR is a global regulator of bile acid metabolism. Two genes, cholesterol 7a-hydroxylase (CYP7A1) and IBABP (Ileal Bile Acid Binding Protein), which are implicated in bile acid biosynthesis and recycling, respectively, are target genes of FXR. FXR is shown to be transcriptionally activated by farnesol metabolites such as farnesol itself and juvenile hormone III. FXR binds to DNA only as a heterodimer with Retinoid X Receptor (RXR).

Long Name

Farnesoid X-activated receptor

Alternate Names

BAR, HRR-1, NR1H4, RIP14

Entrez Gene IDs

9971 (Human); 20186 (Mouse); 60351 (Rat)

Gene Symbol

NR1H4

Additional FXR/NR1H4 Products

Product Documents for Human FXR/NR1H4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FXR/NR1H4 Antibody

For research use only

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