Human G-CSF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2141
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 741250
Product Specifications
Immunogen
E. coli-derived human G-CSF protein
Thr31-Leu204
Accession # P09919
Thr31-Leu204
Accession # P09919
Specificity
Detects human G-CSF by direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human G-CSF Antibody
Detection of G-CSF in U-87 MG (Positive) & MOLT-4 (Negative).
G-CSF was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line (Positive) & absent in MOLT-4 human acute lymphoblastic leukemia cell line (Negative) using Mouse Anti-Human G-CSF Monoclonal Antibody (Catalog # MAB2141) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Human G-CSF ELISA Standard Curve.
Recombinant Human G-CSF protein was serially diluted 2-fold and captured by Mouse Anti-Human G-CSF Monoclonal Antibody (Catalog # MAB2141) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human G-CSF Monoclonal Antibody (MAB11296) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human G-CSF Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when
paired with human anti G-CSF Monoclonal Antibody (Catalog # MAB11296).This
product is intended for assay development on various assay platforms requiring
antibody pairs.
Immunocytochemistry
8 µg/mL
Sample: Immersion fixed U-87 MG human glioblastoma/astrocytoma cell line (Positive) & MOLT-4 human acute lymphoblastic leukemia cell line (Negative)
Sample: Immersion fixed U-87 MG human glioblastoma/astrocytoma cell line (Positive) & MOLT-4 human acute lymphoblastic leukemia cell line (Negative)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: G-CSF
G-CSF
is a pleiotropic cytokine best known for its specific effects on the
proliferation, differentiation, and activation of hematopoietic cells of
the neutrophilic granulocyte lineage. It is produced mainly by
monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma.
Other cell types including fibroblasts, endothelial cells, astrocytes
and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or
TNF-alpha activation. In addition, various carcinoma cell lines and
myeloblastic leukemia cells can express G-CSF constitutively.
In
humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino
acid precursor proteins, have been isolated. Both proteins have a 30
amino acid signal peptide and have identical amino acid sequences except
for a three amino acid insertion (deletion) at the 35th amino acid
residue from the N-terminus of the mature protein. Human G-CSF is 73%
identical at the amino acid level to murine G-CSF and the two proteins
show species cross-reactivity.
In vitro, G-CSF stimulates
growth, differentiation and functions of cells from the neutrophil
lineage. It also has blast cell growth factor activity and can synergize
with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro
functions, G-CSF has been found to play important roles in defense
against infection, in inflammation and repair, and in the maintenance of
steady state hematopoiesis.
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
UniProt
Additional G-CSF Products
Product Documents for Human G-CSF Antibody
Product Specific Notices for Human G-CSF Antibody
For research use only
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