Human GADD45 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5510
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GADD45 alpha
Met1-Arg165
Accession # P24522
Met1-Arg165
Accession # P24522
Specificity
Detects human GADD45 alpha in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human GADD45 alpha Antibody
Detection of Human GADD45 alpha by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with full length human GADD45a. PVDF membrane was probed with 1 µg/mL Human GADD45a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5510) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for GADD45a was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human GADD45 alpha Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line transfected with full length human GADD45 alpha
Sample: 293T human embryonic kidney cell line transfected with full length human GADD45 alpha
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GADD45 alpha
GADD45 alpha (Growth arrest and DNA damage-inducible protein 45 alpha) is a 19 kDa member of the GADD45 family of proteins. It is ubiquitously expressed, and induced by DNA damage. Upon induction, GADD45 alpha binds to PCNA, stimulates DNA excision repair, and initiates G2 cell cycle arrest. Human GADD45 alpha is 165 amino acids (aa) in length. It is reported to dimerize with GADD45-beta and -gamma, and to homo-dimerize, -trimerize and -tetramerize via residues 33‑61 and 133‑165. There is one splice variant that shows a Ser substitution for aa 14‑49. GADD45 alpha is 55% aa identical to GADD45-beta and -gamma, and shares 94% aa identity with mouse GADD45 alpha.
Long Name
Growth Arrest and DNA Damage Gene alpha
Alternate Names
GADD45a
Gene Symbol
GADD45A
UniProt
Additional GADD45 alpha Products
Product Documents for Human GADD45 alpha Antibody
Product Specific Notices for Human GADD45 alpha Antibody
For research use only
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