HEK293 human embryonic kidney cell line transfected with human Galanin R2/GALR2 and eGFP was stained with either (A) Mouse Anti-Human Galanin R2/GALR2 Monoclonal Antibody (Catalog # MAB6544) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Galanin R2 in Human Brain.

Galanin R2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human Galanin R2 Monoclonal Antibody (Catalog # MAB6544) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells of blood vessels. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Galanin R2/GALR2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Galanin R2/GALR2 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galanin R2/GALR2

GALR2 (Galanin Receptor 2) is one of three 54-60 kDa G-protein coupled multipass transmembrane receptors for the Galanin neuropeptide. Galanin is co-expressed with and modulates noradrenaline and serotonin systems. GALR2 is thought to modulate growth and apoptosis. Unlike GALR and GALR3, it is thought to be anti-depressive. GALR2 mRNA is found mainly in the central nervous system (except for cerebral cortex), but also in intestine, heart, kidney, liver and many cancers. The combined extracellular domains of human GALR2 share 82% and 86% amino acid identity with mouse and rat GALR2, respectively.

Long Name

Galanin Receptor Type 2

Alternate Names

GAL2R, GalaninR2, GALNR2, GALR2

Entrez Gene IDs

8811 (Human); 29234 (Rat)

Gene Symbol

GALR2

UniProt

O43603

Additional Galanin R2/GALR2 Products

Product Documents for Human Galanin R2/GALR2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Galanin R2/GALR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Galanin R2/GALR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6544

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Galanin R2/GALR2 Antibodies »

Product Summary for Human Galanin R2/GALR2 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Galanin R2/GALR2 Antibody

Detection of Galanin R2/GALR2 in HEK293 Human Cell Line Transfected with Human Galanin R2/GALR2 and eGFP by Flow Cytometry.