Human gamma-Glutamylcyclotransferase/CRF21 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5086
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human gamma‑Glutamylcyclotransferase/CRF21
Met1-Leu188
Accession # O75223
Met1-Leu188
Accession # O75223
Specificity
Detects human gamma‑Glutamylcyclotransferase/CRF21 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human gamma-Glutamylcyclotransferase/CRF21 Antibody
Detection of Human gamma‑Glutamylcyclotransferase/CRF21 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human gamma-Glutamylcyclotransferase/CRF21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5086) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma-Glutamylcyclotransferase/CRF21 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human gamma-Glutamylcyclotransferase/CRF21 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: gamma-Glutamylcyclotransferase/CRF21
Gamma-glutamylcyclotransferase (GGCT; also Cytochrome c-releasing factor 21, CRF21, C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta-sheets and alpha-helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97‑188. Full-length human CRF21 is 82% aa identical to mouse CRF21.
Long Name
Cytochrome c Releasing Factor 21
Alternate Names
C7orf24, CRF21, gammaGlutamylcyclotransferase, GCTG, GGC, GGCT, LOC79017
Gene Symbol
GGCT
UniProt
Additional gamma-Glutamylcyclotransferase/CRF21 Products
Product Documents for Human gamma-Glutamylcyclotransferase/CRF21 Antibody
Product Specific Notices for Human gamma-Glutamylcyclotransferase/CRF21 Antibody
For research use only
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