Human Gastrokine 1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6395

R&D Systems, part of Bio-Techne
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MAB6395
MAB6395-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 665701

View all Gastrokine 1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Gastrokine 1
Asn35-Asn199
Accession # Q9NS71

Specificity

Detects human Gastrokine 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Gastrokine 1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Gastrokine 1 Antibody

Gastrokine 1 antibody in Human Stomach by Immunohistochemistry (IHC-P).

Gastrokine 1 in Human Stomach.

Gastrokine 1 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Gastrokine 1 Monoclonal Antibody (Catalog # MAB6395) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Gastrokine 1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gastrokine 1

Gastrokine 1 (GKN1; also CA11 and AMP-18) is an 18 kDa member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165 amino acids (aa) in length. Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 54-150) that contains a mitogenic sequence (aa 97-117). There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa 21-185 (aa 35-199 of SwissProt), human Gastrokine 1 shares 65% aa identity with mouse Gastrokine 1.  

Long Name

Take out Column D name

Alternate Names

AMP18, BRICD1, CA11, FOV, Foveolin, GKN1

Entrez Gene IDs

56287 (Human); 66283 (Mouse); 297418 (Rat)

Gene Symbol

GKN1

UniProt

Q9NS71 (Human)

Additional Gastrokine 1 Products

Product Documents for Human Gastrokine 1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Gastrokine 1 Antibody

For research use only

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