Human Gastrokine 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6395
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Gastrokine 1
Asn35-Asn199
Accession # Q9NS71
Asn35-Asn199
Accession # Q9NS71
Specificity
Detects human Gastrokine 1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Gastrokine 1 Antibody
Detection of Human Gastrokine 1 by Western Blot.
Western blot shows lysates of human stomach tissue. PVDF Membrane was probed with 1 µg/mL of Human Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6395) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Gastrokine 1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Gastrokine 1 in Human Stomach.
Gastrokine 1 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6395) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to luminal part of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Gastrokine 1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human stomach subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: Human stomach tissue
Sample: Human stomach tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gastrokine 1
Gastrokine 1 (GKN1; also CA11 and AMP-18) is an 18 kDa member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165 amino acids (aa) in length. Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 54‑150) that contains a mitogenic sequence (aa 97‑117). There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa 21‑185 (aa 35‑199 of SwissProt Q9NS71), human Gastrokine 1 shares 65% aa identity with mouse Gastrokine 1.
Long Name
Take out Column D name
Alternate Names
AMP18, BRICD1, CA11, FOV, Foveolin, GKN1
Gene Symbol
GKN1
UniProt
Additional Gastrokine 1 Products
Product Documents for Human Gastrokine 1 Antibody
Product Specific Notices for Human Gastrokine 1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...