Human GATA-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1779
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976
Met1-Ser413
Accession # P15976
Specificity
Detects human GATA-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with rhGATA-2, rhGATA-5 and rhGATA-6.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human GATA-1 Antibody
Detection of Human GATA-1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL of Human GATA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1779) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-1 at approximately 50 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human GATA-1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and TS1 mouse helper T cell line
Sample: K562 human chronic myelogenous leukemia cell line and TS1 mouse helper T cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-1
References
- Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.
Alternate Names
GATA1
Gene Symbol
GATA1
UniProt
Additional GATA-1 Products
Product Documents for Human GATA-1 Antibody
Product Specific Notices for Human GATA-1 Antibody
For research use only
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