Human GATA-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB26051
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 291106
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Pro135-Ser258
Accession # P23771
Specificity
Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with rhGATA-1, -2, -4, -5, or -6.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GATA-3 Antibody
Detection of Human GATA‑3 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GATA-3 full length (FL) at approximately 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
GATA-3 in MCF-7 Human Cell Line.
GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GATA-3 Monoclonal Antibody (Catalog # MAB26051) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GATA-3 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Western Blot
2 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-3
GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.
Additional GATA-3 Products
Product Documents for Human GATA-3 Antibody
Product Specific Notices for Human GATA-3 Antibody
For research use only
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