Human GATA-3 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2605
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Pro135-Ser258
Accession # P23771
Specificity
Detects human GATA-3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human GATA-3 Biotinylated Antibody
GATA-3 in MCF-7 Human Cell Line.
GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2605) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GATA-3 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human GATA-3
Sample: Recombinant Human GATA-3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-3
Additional GATA-3 Products
Product Documents for Human GATA-3 Biotinylated Antibody
Product Specific Notices for Human GATA-3 Biotinylated Antibody
For research use only
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