Human GCNF/NR6A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3180
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GCNF/NR6A1
Met215-Glu480
Accession # Q15406
Met215-Glu480
Accession # Q15406
Specificity
Detects human and mouse GCNF/NR6A1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human GCNF/NR6A1 Antibody
GCNF/NR6A1 in HepG2 Human Cell Line.
GCNF/NR6A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line (negative staining) using Goat Anti-Human GCNF/NR6A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3180) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human GCNF/NR6A1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human GCNF/NR6A1
Sample: Recombinant Human GCNF/NR6A1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GCNF/NR6A1
Long Name
Germ Cell Nuclear Factor
Alternate Names
NR6A1, RTR
Entrez Gene IDs
2649 (Human)
Gene Symbol
NR6A1
UniProt
Additional GCNF/NR6A1 Products
Product Documents for Human GCNF/NR6A1 Antibody
Product Specific Notices for Human GCNF/NR6A1 Antibody
For research use only
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