Human GDNF Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF212
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Primate - Chlorocebus sabaeus (Green Monkey)
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF
Arg109-Ile211
Accession # P39905
Arg109-Ile211
Accession # P39905
Specificity
Detects human GDNF in ELISAs and Western blots. In sandwich immunoassays, less than 30% cross-reactivity with recombinant rat GDNF is observed and less than 0.1% cross-reativity with recombinant human (rh) BDNF, rhCNTF, rhNeurturin, rh beta-NGF, rhNT-3, and rhNT-4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human GDNF Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human GDNF (Catalog # 212-GD)
Recombinant Rat GDNF (Catalog # 512-GF)
Sample: Recombinant Human GDNF (Catalog # 212-GD)
Recombinant Rat GDNF (Catalog # 512-GF)
Human GDNF Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDNF
Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.
Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
Long Name
Glial Cell line-derived Growth Factor
Alternate Names
ATF, HFB1-GDNF, HGDNF, HSCR3
Gene Symbol
GDNF
UniProt
Additional GDNF Products
Product Documents for Human GDNF Biotinylated Antibody
Product Specific Notices for Human GDNF Biotinylated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...