Human GFER/ALR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7940
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 829812
Product Specifications
Immunogen
E. coli-derived recombinant human GFER/ALR
Arg82-Asp205
Accession # P55789
Arg82-Asp205
Accession # P55789
Specificity
Detects human GFER/ALR in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GFER/ALR Antibody
Detection of Human GFER/ALR by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFER/ALR at approximately 20-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GFER/ALR in Human Liver.
GFER/ALR was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human GFER/ALR Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFER/ALR
Long Name
Growth Factor, Augmenter of Liver Regeneration
Alternate Names
ALR, ERV1, Hepatopoietin, HERV1, HPO
Gene Symbol
GFER
UniProt
Additional GFER/ALR Products
Product Documents for Human GFER/ALR Antibody
Product Specific Notices for Human GFER/ALR Antibody
For research use only
Loading...
Loading...
Loading...
Loading...