Human Ghrelin/Obestatin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8149
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Ghrelin/Obestatin
Gly24-Lys117 (Leu72Met)
Accession # Q9UBU3
Gly24-Lys117 (Leu72Met)
Accession # Q9UBU3
Specificity
Detects human Ghrelin/Obestatin in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse Ghrelin/Obestatin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Ghrelin/Obestatin Antibody
Detection of Human Ghrelin/Obestatin by Western Blot.
Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Ghrelin/Obestatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ghrelin/Obestatin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Ghrelin/Obestatin in Mouse Stomach.
Ghrelin/Obestatin was detected in immersion fixed frozen sections of mouse stomach using Sheep Anti-Human Ghrelin/Obestatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8149) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining is shown at both 60x magnification (upper panel) and 20x magnification (lower panel). Staining was localized to cytoplasm and gastric endocrine cells, respectively. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Ghrelin/Obestatin in Human Stomach.
Ghrelin/Obestatin was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human Ghrelin/Obestatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8149) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of gastric mucosa. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Ghrelin/Obestatin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse stomach and immersion fixed paraffin-embedded sections of human stomach
Sample: Immersion fixed frozen sections of mouse stomach and immersion fixed paraffin-embedded sections of human stomach
Western Blot
2 µg/mL
Sample: Human stomach tissue
Sample: Human stomach tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ghrelin/Obestatin
GHRL (Ghrelin/Obestatin Prepropeptide) is a 117 aa precursor peptide that is processed into three chains, Ghrelin-27 (aa 24-50), Ghrelin-28 (aa24-51) and Obestatin (aa76-98). The prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases. Mature Ghrelin is modified with an N-octanoyl group on serine 3 which is required for receptor binding. Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa. Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs. Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin.
Alternate Names
appetite-regulating hormone, ghrelin, ghrelin/obestatin preprohormone, ghrelin/obestatin prepropeptide, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, MTLRPgrowth hormone secretagogue receptor ligand, obestatin, Protein M46
Entrez Gene IDs
51738 (Human)
Gene Symbol
GHRL
UniProt
Additional Ghrelin/Obestatin Products
Product Documents for Human Ghrelin/Obestatin Antibody
Product Specific Notices for Human Ghrelin/Obestatin Antibody
For research use only
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