Human GIF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8060
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human GIF
Ser19-Tyr417
Accession # P27352
Ser19-Tyr417
Accession # P27352
Specificity
Detects human GIF in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human GIF Antibody
Detection of Human GIF by Western Blot.
Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human GIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8060) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GIF at approx-imately 48-52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human GIF Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human stomach tissue
Sample: Human stomach tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GIF
GIF (Gastric Intrinsic Factor; also better known as IF or Intrinsic Factor) is a 45-55 kDa, secreted glycoprotein member of the eukaryotic cobalamin transport family of proteins. It should not be confused with either GIF/growth inhibitory factor/metallothionein 3, or GIF/glycosylation-inhibiting factor/macrophage migration inhibitory factor/phenylpyruvate tautomerase. GIF shows restricted expression, being essentially limited to parietal and select chief cells of the stomach. GIF binds to haptocorrin-associated cobalamin/VitB12 in the duodenum, whereupon it passes into the ileum and is taken up by a 500 kDa epithelial receptor named cubam, a complex of cubilin and amnionless molecules. Mature human GIF is 399 amino acids (aa) in length (aa 19-417) that contains one principal B12 binding site between aa 365-395. There is one isoform variant that shows a deletion of aa 3-27. Full-length human GIF shares 82% aa sequence identity with mouse GIF.
Long Name
Gastric Intrinsic Factor
Alternate Names
IF, IFMH, INF, TCN3
Gene Symbol
CBLIF
UniProt
Additional GIF Products
Product Documents for Human GIF Antibody
Product Specific Notices for Human GIF Antibody
For research use only
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