Human GIP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8864

R&D Systems, part of Bio-Techne
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MAB8864
MAB8864-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 911205

View all GIP Antibodies »

Product Specifications

Immunogen

GIP Peptide
Tyr1-Asn39
Accession # P09681

Specificity

Detects human GIP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GIP Antibody

GIP antibody in Human Stomach by Immunohistochemistry (IHC-P).

GIP in Human Stomach.

GIP was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human GIP Monoclonal Antibody (Catalog # MAB8864) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human GIP Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Reviewed Applications

Read 1 review rated 5 using MAB8864 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GIP

Gastric inhibitory polypeptide (GIP), also known as the glucose-dependent insulinotropic peptide is a member of the secretin family of hormones. GIP, together with glucagon-like peptide-1 (GLP-1), belongs to the group of metabolic hormones called incretins that stimulate a decrease in blood glucose levels. GIP is derived from a 153-amino acid pro-protein encoded by the GIP gene and circulates as a biologically active 42-amino acid peptide.  Engagement of Gastric inhibitory polypeptide receptors (GIPR) by GIP on pancreatic beta cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose.

GIP (1-39) is an endogenous truncated form of GIP that has been shown to be more potent in stimulating insulin secretion from rat pancreatic islets.

Long Name

Gastric Inhibitory Polypeptide

Alternate Names

Glucose-dependent Insulinotropic Polypeptide, Incretin

Entrez Gene IDs

2695 (Human); 14607 (Mouse); 25040 (Rat)

Gene Symbol

GIP

UniProt

P09681

Additional GIP Products

Product Documents for Human GIP Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GIP Antibody

For research use only

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