Human Girdin Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5345
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Girdin
Asn1510-Leu1824
Accession # Q3V6T2
Asn1510-Leu1824
Accession # Q3V6T2
Specificity
Detects human Girdin in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Girdin Biotinylated Antibody
Detection of Human Girdin by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Girdin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5345) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Girdin at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Girdin Biotinylated Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Girdin
Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196‑425 and 458‑1358), and one phosphoinositide-binding domain (aa 1390‑1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.
Long Name
Girders of Actin Filament
Alternate Names
APE, CCDC88A, GIV, GRDN, HkRP1
Gene Symbol
CCDC88A
UniProt
Additional Girdin Products
Product Documents for Human Girdin Biotinylated Antibody
Product Specific Notices for Human Girdin Biotinylated Antibody
For research use only
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