Human GKAP/DLGAP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7296
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 727425
Product Specifications
Immunogen
E. coli-derived recombinant human GKAP
Lys368-Glu472
Accession # O14490
Lys368-Glu472
Accession # O14490
Specificity
Detects human GKAP in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GKAP/DLGAP1 Antibody
GKAP in Human Brain.
GKAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human GKAP Monoclonal Antibody (Catalog # MAB7296) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human GKAP/DLGAP1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GKAP/DLGAP1
Long Name
Guanylate Kinase-associated Protein
Alternate Names
DAP1, DLGAP1, SAPAP1
Gene Symbol
DLGAP1
UniProt
Additional GKAP/DLGAP1 Products
Product Documents for Human GKAP/DLGAP1 Antibody
Product Specific Notices for Human GKAP/DLGAP1 Antibody
For research use only
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