Human GluR3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11329

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB11329-100
MAB11329-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1059821

View all GluR3 Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # P42263

Specificity

Detects human GluR3 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GluR3 Antibody

Detection of GluR3 in TT (positive) and THP-1 (negative) cells.

GluR3 was detected in immersion fixed TT human medullary thyroid cancer cell line (positive) and absent in THP-1 human acute monocytic leukemia cell line (negative) using Mouse Anti-Human GluR3 Monoclonal Antibody (Catalog # MAB11329) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GluR3 Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: TT human medullary thyroid cancer cell line (positive) and THP-1 human acute monocytic leukemia cell line (negative)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GluR3

GluR3 is a receptor for glutamate. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiological processes. This gene belongs to a family of AMPA receptors.  GluR3 is a gene product of the GRIA3 gene and its pre-mRNA is subject to RNA editing. In humans, 80-90% of GRIA3 transcripts are edited. This allows sustained response to rapid stimuli. There is a tentative link between defective GRIA3 variants and a highly elevated risk of schizophrenia.

References

  1. Entrez Gene: GRIA3 glutamate receptor, ionotropic, AMPA 3.
  2. Seeburg PH, Higuchi M, Sprengel R. "RNA Editing of Brain Glutamate Receptor channels:  Mechanism and Physiology". Brain Res. Brain Res. Rev. 1998, May; 26(2-3):217.
  3. Lomeli H, Mosbacher J, Höger T, Geiner JR, Kuner T, Monyer H, Higuchi M, Bach A, Seeburg PH., "Control of Kinetic Properties of AMPA rREceptor Channels by Nuclear RNA Editing". Science. 1994, Dec; 266(5191):1709.

Long Name

Glutamate Receptor 3

Alternate Names

AMPA3, GLUR-K3, GLURC, GRIA3

Entrez Gene IDs

2892 (Human); 53623 (Mouse); 29628 (Rat)

Gene Symbol

GRIA3

UniProt

P42263 (Human)

Additional GluR3 Products

Product Documents for Human GluR3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GluR3 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.