Detects human Glut3. Recognizes human Glut3 expression on human Glut3-transfected NS0 cells, but not the NS0 control transfectants. No cross-reactivity was observed with transfectants expressing human Glut1 or human Glut2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Glut3 Antibody

Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry.

NS0 mouse myeloma cell line transfected with human Glut3 was stained with Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Glut3 in SH‑SY5Y Human Cell Line.

Glut3 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Glut3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: NS0 mouse myeloma cell line transfected with human Glut3

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line and immersion fixed Caco-2 human colorectal adenocarcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut3

Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members, and is designated SLC2A3 (solute carier family 2, member 3). It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).

References

Vannuci, S.J. et al. (1997) Glia 21:2.
Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
Illsley, N.P. (2000) Placenta 21:14.
Mobasheri, A. (2002) Cell Biol. Int. 26:297.

Long Name

Glucose Transporter Type 3

Alternate Names

SLC2A3

Entrez Gene IDs

6515 (Human); 20527 (Mouse)

Gene Symbol

SLC2A3

UniProt

P11169

Additional Glut3 Products

Product Documents for Human Glut3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Glut3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Glut3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1415

Key Product Details

Species Reactivity

Validated:

Cited:

Applications