Human Glut5 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1349
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2A Clone # 195205
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Glut5
Met1-Gln501
Accession # P22732
Met1-Gln501
Accession # P22732
Specificity
Detects human Glut5. Stains human Glut5-transfected NS0 cells; also detects Glut5 on human microglia cells (Sasaki, A. et al. (2003) Neurosci. Lett. 338(1):17).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Glut5 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: Human Glut5 transfected NS0 cells
Sample: Human Glut5 transfected NS0 cells
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small intestine and skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human small intestine and skeletal muscle
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glut5
Glut5 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains, and isexpressed at variable levels in many tissues. Glut5 is expressed at highest levels insmall intestine and at lower levels in kidney, testis, skeletal muscle and adipose tissue (1, 2). Glut5 transports fructose in intestine, testis and other tissues.
References
- Kayano, T. et al. (1990) J. Biol. Chem. 265(22):13276.
- Corpe, C.P. (2002) Biochem. Biophys. Acta. 1576(1):191.
Long Name
Glucose Transporter Type 5
Alternate Names
SLC2A5
Gene Symbol
SLC2A5
UniProt
Additional Glut5 Products
Product Documents for Human Glut5 Biotinylated Antibody
Product Specific Notices for Human Glut5 Biotinylated Antibody
For research use only
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