Human Glutathione S-Transferase mu 1/GSTM1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6894

R&D Systems, part of Bio-Techne
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MAB6894
MAB6894-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 842516

View all Glutathione S-Transferase mu 1/GSTM1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glutathione S‑Transferase mu 1/GSTM1
Met1-Lys218
Accession # P09488

Specificity

Detects human Glutathione S‑Transferase mu 1/GSTM1 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Glutathione S-Transferase mu 1/GSTM1 Antibody

Detection of Human Glutathione S-Transferase mu 1/GS antibody by Western Blot.

Detection of Human Glutathione S‑Transferase mu 1/GS by Western Blot.

Western blot shows Recombinant Human GSTM1 (Catalog # 6894-GT, 3 ng/lane) and lysates of human adrenal gland tissue and human stomach tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glutathione S-Transferase mu 1/GS Monoclonal Antibody (Catalog # MAB6894) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase mu 1/GS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Glutathione S-Transferase mu 1/GSTM1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human GSTM1 (Catalog # 6894-GT, 3 ng/lane) and lysates of human adrenal gland tissue and human stomach tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase mu 1/GSTM1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).

References

  1. Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
  2. Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
  3. Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
  4. Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
  5. Mo, Z. et al. (2009) Prostate. 69:662.
  6. Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
  7. Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.

Alternate Names

Glutathione STransferase mu 1, GST1, GSTM1, GTH4, GTM1

Entrez Gene IDs

2944 (Human)

Gene Symbol

GSTM1

UniProt

P09488

Additional Glutathione S-Transferase mu 1/GSTM1 Products

Product Documents for Human Glutathione S-Transferase mu 1/GSTM1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Glutathione S-Transferase mu 1/GSTM1 Antibody

For research use only

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