Human Glycine N-methyltransferase/GNMT Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6526
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Glycine N-methyltransferase/GNMT
Met1-Asp295
Accession # Q14749
Met1-Asp295
Accession # Q14749
Specificity
Detects human Glycine N-methyltransferase/GNMT in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Glycine N-methyltransferase/GNMT Antibody
Detection of Human Glycine N-methyltransferase/GNMT by Western Blot.
Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Glycine N-methyltransferase/GNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6526) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glycine N-methyltransferase/GNMT at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Glycine N-methyltransferase/GNMT Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Capan‑1 human pancreatic adenocarcinoma cell line
Sample: Capan‑1 human pancreatic adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glycine N-Methyltransferase/GNMT
GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT.
Alternate Names
Glycine NMethyltransferase, GNMT
Gene Symbol
GNMT
UniProt
Additional Glycine N-Methyltransferase/GNMT Products
Product Documents for Human Glycine N-methyltransferase/GNMT Antibody
Product Specific Notices for Human Glycine N-methyltransferase/GNMT Antibody
For research use only
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