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Human Glycophorin A Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM12281

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # R10

Product Specifications

Immunogen

Abelson mouse leukemia virus-induced human pre-B tumor cells

Specificity

Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Glycophorin A Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: TF-1 human erythroleukemic cell line

Immunohistochemistry

Rearden, A. et al. (1985) Mol. Immunol. 22:369., San Miguel, J.F. et al. (1985) Br. J. Haematol. 59:297.

Immunoprecipitation

Blanchard, D. et al. (1983) Biochem. J. 213:399.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycophorin A

Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).

References

  1. Greaves, M.F. et al. (1983) Blood 61(4):645.

Alternate Names

CD235a, GPA, GYPA

Entrez Gene IDs

2993 (Human)

Gene Symbol

GYPA

Additional Glycophorin A Products

Product Documents for Human Glycophorin A Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Glycophorin A Biotinylated Antibody

For research use only

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