Human Goosecoid Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4086
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Goosecoid
Gly48-Arg159
Accession # P56915
Gly48-Arg159
Accession # P56915
Specificity
Detects human Goosecoid in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Goosecoid Antibody
Goosecoid in BG01V Human Embryonic Stem Cells.
Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human Goosecoid Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to mesoderm with 100 ng/mL recombinant human EGF Activin A (Catalog # 338-AC-005) for 5 days under low serum conditions
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to mesoderm with 100 ng/mL recombinant human EGF Activin A (Catalog # 338-AC-005) for 5 days under low serum conditions
Western Blot
0.1 µg/mL
Sample: Recombinant Human Goosecoid
Sample: Recombinant Human Goosecoid
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Goosecoid
Goosecoid (GSC) is a 28 kDa member of the paired homeobox family. Human Goosecoid is 257 amino acids (aa) in length, and contains one DNA-binding homeodomain (aa 160‑219). Goosecoid appearance correlates with the start of gastrulation and the development of both head/neck and upper body limb mesenchyme. It also appears abnormally in some tumors where it is associated with an epithelial-to-mesenchymal transition. Human Goosecoid shares 97% aa sequence identity with mouse Goosecoid.
Alternate Names
GSC
Gene Symbol
GSC
UniProt
Additional Goosecoid Products
Product Documents for Human Goosecoid Antibody
Product Specific Notices for Human Goosecoid Antibody
For research use only
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