Detects human GPR111 in direct ELISAs. Stains human GPR111 HEK293-transfected cells and does not stain irrelevant non-GPR111 transfectants by Flow Cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human GPR111 Antibody

Detection of Human GPR111 by Western Blot.

Western blot shows lysates of CHO Chinese hamster ovary cell line mock transfected or tranfected with human GPR111. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR111 Monoclonal Antibody (Catalog # MAB64941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GPR111 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of GPR111 in HEK293 Human Cell Line Transfected with Human GPR111 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) GPR111 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human GPR111 Monoclonal Antibody (Catalog # MAB64941) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GPR111 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human GPR111 and eGFP

Western Blot

2 µg/mL
Sample: CHO Chinese hamster ovary cell line tranfected with human GPR111

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR111

GPR111 (G-protein coupled receptor 111; also PRG20) is a 71 kDa (predicted), seven transmembrane (TM) member of the GPR-2 family, LN-7TM subfamily of molecules. It is reportedly expressed in lung, mammary gland and diencephalon. Human GPR111 is 642 amino acids (aa) in length. It contains an extended N-terminal extracellular region with a mucin like stalk (aa 1-383), followed by a series of seven TM domains and a short C-terminal cytoplasmic tail. The N-terminus possesses a GPS (GPCR proteolytic site) (aa 324-368) that likely generates a soluble cleavage product. GPR111 is considered an adhesion-type GPCR, and as such, is expected to form dimers, if not oligomers . There is one potential splice variant for GPR111. It shows a 92 aa substitution for aa 1-24 coupled to a 19 aa substitution for aa 622-642. Over aa 19-375, human GPR111 shares 68% aa identity with mouse GPR111.

Long Name

G-Protein-Coupled Receptor 111

Alternate Names

PGR20

Entrez Gene IDs

222611 (Human); 435529 (Mouse); 301269 (Rat)

Gene Symbol

ADGRF2

UniProt

Q8IZF7 (Human)

Additional GPR111 Products

Product Documents for Human GPR111 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR111 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPR111 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB64941

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all GPR111 Antibodies »

Product Summary for Human GPR111 Antibody

Immunogen

Specificity