Human GPR116 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11248
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1055814
Product Specifications
Immunogen
E. coli-expressed recombinant Human GPR116 extracellular domain.
His643-Asn945
His643-Asn945
Specificity
Detects Human GPR116 in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GPR116 Antibody
Detection of GPR116 in Human Lung.
GPR116 was detected in immersion fixed paraffin-embedded sections of Human Lung using Mouse Anti-Human GPR116 Monoclonal Antibody (Catalog # MAB11248) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human GPR116 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Lung.
Sample: Immersion fixed paraffin-embedded sections of Human Lung.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR116
Long Name
G Protein-coupled Receptor 116
Alternate Names
FLJ90640, G protein-coupled receptor 116, Ig-Hepta homolog, KIAA0758DKFZp564O1923, KPG_001, probable G-protein coupled receptor 116
Gene Symbol
ADGRF5
Additional GPR116 Products
Product Documents for Human GPR116 Antibody
Product Specific Notices for Human GPR116 Antibody
For research use only
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