HEK293 human embryonic kidney cell line transfected with (A) human GPR158 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR158 Monoclonal Antibody (Catalog # MAB102861) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

GPR158 in T47D Human Cell Line.

GPR158 was detected in immersion fixed T47D human breast cancer cell line using Mouse Anti-Human GPR158 Monoclonal Antibody (Catalog # MAB102861) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human GPR158 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human GPR158 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed T47D human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR158

G-protein coupled receptor 158 (GPR158) is a receptor belonging to the Class C GPCR family. It lacks the extracellular Venus flytrap module characteristic of the known members of that family and instead contains two other elements that are not typical of the class: a calcium-binding EGF-like domain and a leucine repeat region (1, 2). The mature extracellular domain of human GPR158 contains 393 amino acids (aa) and shares 89% identity with both mouse and rat GPR158. GPR158 is expressed at the highest level in the brain, but also in a variety of other tissues including retina, spleen, liver and lung (3). GPR158 was originally identified in functional screens linked with biological stress and has been implicated in the osteocalcin effect on cognitive processes in the brain (4, 5), and glaucoma and cancer in the periphery (4, 6).

References

Jingami, H. et al. (2003) Curr. Opin. Neurobiol. 13:271.
Bjarnadóttir, T.K et al. (2005) Gene. 362:70.
Orlandi, C. et al. (2012) J. Cell Biol. 197:711.
Itakura, T. et al. (2019) J. Ocul. Pharmacol. Ther. 35:203.
Khrimian, L. et al. (2017) J. Exp. Med. 214:2859.
Fenner, A. (2015) Nat. Rev. Urol. 12:182.

Alternate Names

KIAA1136, RP11-59G22.1

Entrez Gene IDs

57512 (Human); 241263 (Mouse); 291352 (Rat)

Gene Symbol

GPR158

UniProt

Q5T848

Additional GPR158 Products

Product Documents for Human GPR158 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR158 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPR158 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB102861

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GPR158 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPR158 Antibody

Detection of GPR158 in HEK293 Human Cell Line Transfected with Human GPR158 and eGFP by Flow Cytometry.